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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a critical position in selling cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two additional splice variants had been documented but comprehensive nucleotide sequence information and facts is not really but readily available.

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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged because of the antibody.

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This information outlines additional attainable optimization of mobile and immunoassay parameters to be sure the best possible final results are...

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This guidebook outlines even more achievable optimization of mobile and immunoassay parameters to make certain the absolute best results are...

Antibodies certain to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.

Target Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins show unique expression and degradation styles which add to your temporal coordination of each and every mitotic celebration. Cyclin E varieties a complex with and features as being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S section boundary which is degraded as cells progress as a result of S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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